Functional Characterization of Dense Granule Proteins in Toxoplasma gondii RH Strain Using CRISPR-Cas9 System
نویسندگان
چکیده
منابع مشابه
Bacterial Production of Dense Granule Antigen GRA8 of Toxoplasma gondii
Background: Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. Methods: GRA8 comple...
متن کاملEfficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9
Toxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the T. gondii genome remains labor intensive, and is often restricted to lab-adapted strains or lines carrying mutations that enable selection. Here, we use the RNA-guided Cas9 nuclease to efficiently generate ...
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Toxoplasma gondii is an obligatory intracellular apicomplexan protozoan which can infect any warm-blooded animal and causes severe diseases in immunocompromised individuals or infants infected in utero. The survival and success of this parasite require that it colonizes the host cell, avoids host immune defenses, replicates within an appropriate niche, and exits the infected host cell to spread...
متن کاملbacterial production of dense granule antigen gra8 of toxoplasma gondii
background: dense granule antigens (gra antigens) of toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. previous studies reported expression of amino terminal gra8 protein in fusion with large tags such as glutathione-s-transferase. the present study aimed to produce soluble full length immunogenic gra8 in bacteria. methods: gra8 comple...
متن کاملBacterial production of dense granule antigen GRA8 of Toxoplasma gondii.
BACKGROUND Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. METHODS GRA8 comple...
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ژورنال
عنوان ژورنال: Frontiers in Cellular and Infection Microbiology
سال: 2018
ISSN: 2235-2988
DOI: 10.3389/fcimb.2018.00300